Goat anti-biotin, ALP conjugated
AS10 1452 Clonality: Polyclonal Host: Goat Reactivity: biotin
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Product Information
Immunogen
Biotin
Host
Goat
Clonality
Polyclonal
Purity
Affinity purified IgG
Format
Liquid
Quantity
1 mg
Storage
Non-diluted antibody is stable for 4 years at 2-8°C, For storage at -20°C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity, Prepare working dilutions prior to use and then discard, Shelf life is 1 year from date of receipt, Prepare working dilution prior to use and then discard
Recommended dilution
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Reactivity
Application examples
Additional information
Additional information
Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,50 mg/ml (E 1% at 280 nm = 13,0), Antibody is supplied in 30 mM triethanolamine, pH 7,2,5 mM magnesium chloride, 0,1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free, Contains 0,05% (w/v) sodium azide as preservative of bacterial growth
Background
Background
Goat anti-biotin is a secondary antibody conjugated to ALP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.
Product citations
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