Goat anti-biotin, FITC conjugated
AS10 1453 | Clonality:Polyclonal | Host: Goat | Reactivity: biotin
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Purity Affinity purified IgG
Quantity 1 mg
Storage Store at 2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Recommended dilution The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications.
Additional information Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
Background Goat anti-biotin is a secondary antibody conjugated to FITC, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm.