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Goat anti-Dog IgG (H&L), HRP conjugated

AS16 3127 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgG (H&L)

Goat anti-Dog IgG (H&L), HRP conjugated  in the group Secondary Antibodies / Anti-Dog / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS16 3127)

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Product Information

Immunogen

Purified normal IgG isolated from pooled dog serum

Host Goat
Clonality Polyclonal
Purity Protein G purified IgG
Format Lyophilized in PBS, pH 7.2
Quantity 10 mg
Reconstitution For reconstitution add 1 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in 4°C up to 7 days.
Tested applications Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
Recommended dilution 1 : 1000-1 : 10 000 (ELISA), 1 : 100 and 1 : 500 (ICC), (IHC). The optimal working dilution should be established by titration by the end user. Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal.

Reactivity

Confirmed reactivity Dog IgG
Not reactive in

This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.

Application examples

Additional information

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

No preservative added.

The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.


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Background

Background

Goat anti-Dog IgG (H&L), HRP conjugated  is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.

Product citations

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