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Goat anti-Dog IgM Fc specific, Biotin conjugated

AS16 3129 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgM Fc specific

Goat anti-Dog IgM Fc specific, Biotin conjugated  in the group Secondary Antibodies / Anti-Dog / Biotinylated at Agrisera AB (Antibodies for research) (AS16 3129)



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Product Information

Immunogen

Purified normal IgM isolated from pooled dog serum

Host Goat
Clonality Polyclonal
Purity Protein G purified goat IgG in PBS pH 7.2.
Format Lyophilized
Quantity 10 mg
Reconstitution For reconstitution, add 1 ml of sterile distilled water, spin down to remove any insoluble particles.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.  Working dilutions should be prepared by adding sterile PBS pH 7.2. Working dilutions should be stored at 4°C, not frozen, and preferably used during the same day. If any precipitation occurs, remove it by centrifugation.
Tested applications Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
Recommended dilution 1 : 500-1: 4000 (ELISA), 1 : 100-1: 250 (ICC), (IHC), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal

Reactivity

Confirmed reactivity Dog IgM, Fc part
Not reactive in

This antibody does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion

Application examples

Additional information

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail.

No preservative added.

Related products

Background

Background

Goat anti-Dog IgM Fc, Biotin conjugated is a secondary antibody which binds to dog IgM Fc part in immunological assays.

Product citations

immunogen:

Purified normal IgM isolated from pooled dog serum

Reconstitution: For reconstitution, add 1 ml of sterile distilled water, spin down to remove any insoluble particles.
Host: Goat
Clonality: Polyclonal
Purity: Protein G purified goat IgG in PBS pH 7.2.
Format: Lyophilized
Quantity: 10 mg
storage: Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.  Working dilutions should be prepared by adding sterile PBS pH 7.2. Working dilutions should be stored at 4°C, not frozen, and preferably used during the same day. If any precipitation occurs, remove it by centrifugation.
tested applications: Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
recommended dilution: 1 : 500-1: 4000 (ELISA), 1 : 100-1: 250 (ICC), (IHC), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal
Confirmed reactivity: Dog IgM, Fc part
not reactive in:

This antibody does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion

additional information (application):

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail.

No preservative added.

background:

Goat anti-Dog IgM Fc, Biotin conjugated is a secondary antibody which binds to dog IgM Fc part in immunological assays.

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