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Goat anti-Dog IgM Fc specific, Biotin conjugated

AS16 3129 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgM Fc specific

Goat anti-Dog IgM Fc specific, Biotin conjugated  in the group Secondary Antibodies / Anti-Dog / Biotinylated at Agrisera AB (Antibodies for research) (AS16 3129)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

Purified normal IgM isolated from pooled dog serum

Host Goat
Clonality Polyclonal
Purity Protein G purified IgG
Format Lyophilized in PBS, pH 7.2
Quantity 1 ml
Reconstitution For reconstitution add 1 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in 4°C up to 7 days.
Tested applications Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
Recommended dilution 1 : 500-1: 4000 (ELISA), 1 : 100-1: 250 (ICC), (IHC). The optimum working dilution should be established by titration by end user. Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal.

Reactivity

Confirmed reactivity Dog IgM, Fc part
Not reactive in

This antibody does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion

Application examples

Additional information

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail.

No preservative added.

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Background

Background

Goat anti-Dog IgM Fc, Biotin conjugated is a secondary antibody which binds to dog IgM Fc part in immunological assays.

Product citations

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