Goat anti-Dog IgM Fc specific, Biotin conjugated

Name: Goat anti-Dog IgM Fc specific, Biotin conjugated
Brand: IKP
SKU: AS16 3129
Price: 546 EUR
Availability: InStock

AS16 3129 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgM Fc specific

Goat anti-Dog IgM Fc specific, Biotin conjugated in the group Secondary Antibodies / Anti-Dog / Biotinylated at Agrisera AB (Antibodies for research) (AS16 3129)

Product Information

Immunogen

Purified normal IgM isolated from pooled dog serum

Host Goat

Clonality Polyclonal

Purity Protein G purified goat IgG in PBS pH 7.2.

Format Lyophilized

Quantity 10 mg

Reconstitution For reconstitution, add 1 ml of sterile distilled water, spin down to remove any insoluble particles.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be prepared by adding sterile PBS pH 7.2. Working dilutions should be stored at 4°C, not frozen, and preferably used during the same day. If any precipitation occurs, remove it by centrifugation.

Tested applications Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)

Recommended dilution 1 : 500-1: 4000 (ELISA), 1 : 100-1: 250 (ICC), (IHC), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal

Reactivity

Confirmed reactivity Dog IgM, Fc part

Not reactive in

This antibody does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion

Application examples

Additional information

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail.

No preservative added.

Background

Goat anti-Dog IgM Fc, Biotin conjugated is a secondary antibody which binds to dog IgM Fc part in immunological assays.

Product citations

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immunogen:	Purified normal IgM isolated from pooled dog serum
Reconstitution:	For reconstitution, add 1 ml of sterile distilled water, spin down to remove any insoluble particles.
Host:	Goat
Clonality:	Polyclonal
Purity:	Protein G purified goat IgG in PBS pH 7.2.
Format:	Lyophilized
Quantity:	10 mg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be prepared by adding sterile PBS pH 7.2. Working dilutions should be stored at 4°C, not frozen, and preferably used during the same day. If any precipitation occurs, remove it by centrifugation.
tested applications:	Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
recommended dilution:	1 : 500-1: 4000 (ELISA), 1 : 100-1: 250 (ICC), (IHC), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal
Confirmed reactivity:	Dog IgM, Fc part
not reactive in:	This antibody does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion
additional information (application):	Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail. No preservative added.
background:	Goat anti-Dog IgM Fc, Biotin conjugated is a secondary antibody which binds to dog IgM Fc part in immunological assays.