Goat anti-Dog IgM Fc specific, HRP conjugated
AS16 3131 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgM Fc specific
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Product Information
Immunogen
Purified normal IgM isolated from pooled dog serum
Host
Goat
Clonality
Polyclonal
Purity
Protein G purified IgG
Format
Lyophilized in PBS, pH 7.2
Quantity
10 mg
Reconstitution
For reconstitution add 1 ml of sterile destilled water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in 4°C up to 7 days.
Tested applications
Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
Recommended dilution
1 : 100-1:500 (ICC), (IHC), 1 : 500-1 :2000 (ELISA). The optimum working dilution should be established by titration by end user. Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal.
Reactivity
Confirmed reactivity
Dog IgM, Fc part
Not reactive in
This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Application examples
Additional information
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail.
No preservative added.
Background
Background
Goat anti-Dog IgM Fc, HRP conjugated is a secondary antibody which binds to dog IgM Fc part, in immunological assays.
Product citations
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