Goat anti-Dog IgM Fc specific, Unconjugated

AS16 3128 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgM

Product Information

Immunogen

Purified normal IgM isolated from pooled dog serum

Host Goat

Clonality Polyclonal

Purity Protein G purified IgG

Format Lyophilized

Quantity 1 ml

Reconstitution For reconstitution add 1 ml of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in 4°C up to 7 days.

Tested applications immuniprecipitation (IP)

Recommended dilution

Immunoprecipitation. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Precipitin titre 1:64 when tested against pooled normal dog serum in agar-block immunodiffusion titration.

Reactivity

Confirmed reactivity Dog IgM, Fc part

Not reactive in

The antibody does not cross-react with any other component of the dog immunoglobulin system.

Application examples

Additional information

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

No preservative added.