Goat anti-Human IgE heavy (epsilon chain), HRP conjugated
AS10 754 Clonality: Polyclonal Host: Goat Reactivity: Human IgE heavy (epsilon chain)
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Product Information
Immunogen
purified human IgE
Host
Goat
Clonality
Polyclonal
Purity
Affinity purified goat IgG
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage
Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
The optimal working dilution should be determined by the investigator.
Reactivity
Confirmed reactivity
Human IgE heavy (epsilon chain)
Predicted reactivity
Human IgE Heavy (epsilon chain)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
The amount of cross-reactivity to human IgG/M/A has been tested, and it is very low. During manufacturing of this product, cross-reactivity to other IgG is removed. Please see the percentage of measured cross-reactivity to other human immunoglobulins below: Human IgG: 0.12 % Human IgA: 0.09 % Human IgM: 0.17 %No reactivity is observed to the light chains or non-immunoglobulin human serum proteins.
Background
Background
Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to HRP which binds to human IgE heavy (epsilon chain) in immunological assays.
Product citations
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