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Goat anti-Human IgE heavy (epsilon chain), HRP conjugated

AS10 754 Clonality: Polyclonal    Host: Goat  Reactivity: Human IgE heavy (epsilon chain)

Goat anti-Human IgE heavy (epsilon chain), HRP conjugated  in the group Secondary Antibodies / Anti-Human / HRP (horse radish peroxidase) / IgE at Agrisera AB (Antibodies for research) (AS10 754)

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Product Information

Immunogen

purified human IgE

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution The optimal working dilution should be determined by the investigator.

Reactivity

Confirmed reactivity Human IgE heavy (epsilon chain)
Predicted reactivity Human IgE Heavy (epsilon chain)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

The amount of cross-reactivity to human IgG/M/A has been tested, and it is very low. During manufacturing of this product, cross-reactivity to other IgG is removed. Please see the percentage of measured cross-reactivity to other human immunoglobulins below: Human IgG: 0.12 % Human IgA: 0.09 % Human IgM: 0.17 %

No reactivity is observed to the light chains or non-immunoglobulin human serum proteins.

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Background

Background

Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to HRP which binds to human IgE heavy (epsilon chain) in immunological assays.

Product citations

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