Goat anti-Human IgE heavy (epsilon chain), HRP conjugated

AS10 754 Clonality: Polyclonal Host: Goat Reactivity: Human IgE heavy (epsilon chain)


product information
Background		Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to HRP which binds to human IgE heavy (epsilon chain) in immunological assays.
Immunogen		purified human IgE
Host		Goat
Clonality		Polyclonal
Clone
Purity		Affinity purified goat IgG
Format		Lyophilized
Quantity		1 mg
Reconstitution		For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications		ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related products		antibodies to human immunoglobulins
Additional information		HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase The amount of cross-reactivity to human IgG/M/A has been tested, and it is very low. During manufacturing of this product, cross-reactivity to other IgG is removed. Please see the percentage of measured cross-reactivity to other human immunoglobulins below: Human IgG: 0.12 % Human IgA: 0.09 % Human IgM: 0.17 %

application information
Recommended dilution		The optimal working dilution should be determined by the investigator.
Expected \| apparent MW
Confirmed reactivity		Human IgE heavy (epsilon chain)
Predicted reactivity		Human IgE Heavy (epsilon chain)
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		No reactivity is observed to the light chains or non-immunoglobulin human serum proteins.
Selected references		to be added when available

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