Goat anti-Human IgE heavy (epsilon chain), HRP conjugated

Product no: AS10 754

AS10 754 Clonality: Polyclonal    Host: Goat  Reactivity: Human IgE heavy (epsilon chain)

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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    purified human IgE

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: The optimal working dilution should be determined by the investigator
  • Reactivity
  • Confirmed reactivity: Human IgE heavy (epsilon chain)
    Predicted reactivity: Human IgE Heavy (epsilon chain)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

    0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

    The amount of cross-reactivity to human IgG/M/A has been tested, and it is very low. During manufacturing of this product, cross-reactivity to other IgG is removed. Please see the percentage of measured cross-reactivity to other human immunoglobulins below: Human IgG: 0.12 % Human IgA: 0.09 % Human IgM: 0.17 %
    Additional information (application): No reactivity is observed to the light chains or non-immunoglobulin human serum proteins
  • Background
  • Background:

    Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to HRP which binds to human IgE heavy (epsilon chain) in immunological assays.

  • Protocols
  • Antibody protocols
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