Goat anti-Human IgE heavy (epsilon chain), HRP conjugated
AS10 754 Clonality: Polyclonal Host: Goat Reactivity: Human IgE heavy (epsilon chain)
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purified human IgE
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidaseThe amount of cross-reactivity to human IgG/M/A has been tested, and it is very low. During manufacturing of this product, cross-reactivity to other IgG is removed. Please see the percentage of measured cross-reactivity to other human immunoglobulins below: Human IgG: 0.12 % Human IgA: 0.09 % Human IgM: 0.17 %
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins.
Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to HRP which binds to human IgE heavy (epsilon chain) in immunological assays.