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Goat anti-Human IgE heavy (epsilon chain), ALP conjugated, min. cross-reactivity to bovine/mouse/rabbit serum

AS10 756 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Human IgE heavy (epsilon chain)

Goat anti-Human IgE heavy (epsilon chain), ALP conjugated, min. cross-reactivity to bovine/mouse/rabbit serum in the group Secondary Antibodies / Anti-Human / ALP (alkaline phosphatase) / IgE at Agrisera AB (Antibodies for research) (AS10 756)

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Product Information

Immunogen

purified human IgE

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Liquid
Quantity 0.5 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Human IgE heavy (epsilon chain)
Predicted reactivity Human IgE Heavy (epsilon chain)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

No reactivity is observed to light chains or non-IgE human serum proteins based in immunoelectrophoresis

no reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis

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Background

Background

Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgE heavy (epsilon chain) in immunological assays.

Product citations

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