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Goat anti-Human IgE (epsilon chain), DyLightŪ 633 conjugated

AS12 2147 | Clonality: Polyclonal  |  Host: Goat | Reactivity: Human IgE (ε chain)

Goat anti-Human IgE (epsilon chain), DyLightŪ 633 conjugated in the group Secondary Antibodies / Anti-Human / Fluorescent / IgE at Agrisera AB (Antibodies for research) (AS12 2147)

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Product Information

Immunogen Purified human IgE (?-chain)
Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage Store lyophilized material at 2-8°C, Product is stable for 4 weeks at 2-8°C after rehydration,For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity, For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol, Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol, Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming
Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Additional information

Additional information Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative

Based on immunoelectrophoresis, this antibody reacts with:  (ε) chains on human IgE (µ chain).

No reactivity is observed to:non-immunoglobulin human serum proteinslight chains on all human immunoglobulins.

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Background

Background

Goat anti-human IgE (ε chain) - DyLight® 633 Conjugated is a secondary antibody conjugated to  DyLight® 633, which binds to human IgE (ε chain) in immunological assays. 

DyLight® 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase human IgE.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

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