Goat anti-Human IgE (epsilon chain), DyLight® 650 conjugated

Product no: AS12 2269

AS12 2269 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgE (ε chain)

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  • Product Info
  • Immunogen:

    purified human IgE (epsilon chain)

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA), Flow cytometry (FlowCyt), Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: 1 : 20 -1 : 2000 (ELISA), (FlowCyt), (IF), (WB)
  • Additional Information
  • Additional information:

    Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

    Based on immunoelectrophoresis, this antibody reacts with: heavy (ε) chains on human IgE (ε chain)

    No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins

  • Background
  • Background:

    Goat anti-human IgE (ε chain) - DyLight®650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to human IgE (ε chain) in immunological assays.

    DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase human IgE (ε chain).

    DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Antibody protocols
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