Goat anti-Human IgE (epsilon chain), DyLight® 650 conjugated

Name: Goat anti-Human IgE (epsilon chain), DyLight® 650 conjugated
SKU: AS12 2269
Price: 246 EUR
Availability: InStock

AS12 2269 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgE (ε chain)

Goat anti-Human IgE (epsilon chain), DyLight® 650 conjugated in the group Secondary Antibodies / Anti-Human / Fluorescent / IgE at Agrisera AB (Antibodies for research) (AS12 2269)

Product Information

Immunogen

purified human IgE (epsilon chain)

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Tested applications ELISA (ELISA), Flow cytometry (FlowCyt), Immunofluorescence (IF), Western blot (WB)

Recommended dilution 1 : 20 -1 : 2000 (ELISA), (FlowCyt), (IF), (WB)

Reactivity

Application examples

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (ε) chains on human IgE (ε chain)

No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins

Background

Goat anti-human IgE (ε chain) - DyLight®650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to human IgE (ε chain) in immunological assays.

DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase human IgE (ε chain).

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

immunogen:	purified human IgE (epsilon chain)
Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified goat IgG.
Format:	Lyophilized
Quantity:	1 mg
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications:	ELISA (ELISA), Flow cytometry (FlowCyt), Immunofluorescence (IF), Western blot (WB)
recommended dilution:	1 : 20 -1 : 2000 (ELISA), (FlowCyt), (IF), (WB)
additional information:	Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with: heavy (ε) chains on human IgE (ε chain) No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins
background:	Goat anti-human IgE (ε chain) - DyLight®650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to human IgE (ε chain) in immunological assays. DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase human IgE (ε chain). DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

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