Goat anti-Human IgE (epsilon chain), DyLight® 800 conjugated

AS12 2393 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgE (ε chain)

Product Information

Immunogen

purified human IgE (epsilon chain)

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (ε) chains on human IgE

No reactivity is observed to: non-immunoglobulin human serum proteins, light chains on all human immunoglobulins

Background

Goat anti-human IgE (ε chain), DyLight®800 Conjugated is a secondary antibody conjugated to DyLight® 800, which binds to human IgE (ε chain) in immunological assays.

DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Human IgE

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

Selected references Plundrich et al. (2019). Binding of peanut allergen Ara h 2 with Vaccinium fruit polyphenols. Food Chemistry Vol 284, 30 June 2019, Pages 287-295.

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