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Goat anti-Human IgG Fc, ALP conjugated

AS10 787 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Human IgG Fc (two heavy chains with constant domains)

Goat anti-Human IgG Fc, ALP conjugated  in the group Secondary Antibodies / Anti-Human / ALP (alkaline phosphatase) / IgG at Agrisera AB (Antibodies for research) (AS10 787)

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Product Information

Immunogen

Purified human IgG

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Liquid
Quantity 1 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Human IgG Fc (two heavy chains with constant domains)
Predicted reactivity Human IgG Fc (two Heavy chains with constant domains)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.

No reactivity is observed with the light chains on human immunoglobulins and non-immunoglobulin human serum proteins and human IgG F(ab)'2 fragment based in immunoelectrophoresis.

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Background

Background

Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.

Product citations

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