Goat anti-Human IgG Fc, F(ab)'2 fragment, ALP conjugated, min, cross-reactivity to bovine/mouse/rabbit serum

Product no: AS10 813

AS10 813 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Human IgG Fc (two heavy chains with constant domains)

10 % until end of 2023. Use discount code: Conj10

246
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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    Purified human IgG

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Liquid
    Quantity: 0,5 mg
    Storage: Non-diluted antibody is stable for 4 years at 2-8C. For storage at -20C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
  • Reactivity
  • Confirmed reactivity: Human IgG Fc (two heavy chains with constant domains)
    Predicted reactivity: Human IgG Fc (two Heavy chains with constant domains)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
    Additional information (application):

    This antibody reacts with the heavy (gamma) chains on human IgG based on immunoelectrophoresis.

    No reactivity is observed with the light chains on human immunoglobulins, or non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins or mouse and rabbit IgG based on immunoelectrophoresis.

  • Background
  • Background:

    Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.

  • Protocols
  • Antibody protocols
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