Goat anti-Human IgG Fc, F(ab)'2 fragment, HRP conjugated, min, cross-reactivity bovine/mouse/rabbit serum
AS10 817 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG Fc (two heavy chains with constant domains)
10 % until end of 2023. Use discount code: Conj10
- Product Info
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Immunogen: Purified human IgG
Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 0,5 mg Reconstitution: For reconstitution add 0,55 ml of sterile water Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Tested applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: The optimal working dilution should be determined by the investigator - Reactivity
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Confirmed reactivity: Human IgG Fc (two heavy chains with constant domains) Predicted reactivity: Human IgG Fc (two Heavy chains with constant domains) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Additional information (application): This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.
No reactivity is observed with the light chains on human immunglobulins or non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins or IgG F(ab)' fragment based on immunoelectrophoresis.
The antibody ahs been absorbed against solid phase normal bovine, mouse and rabbit serum in addition to mouse and rabbit IgG.
- Background
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Background: Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
- Protocols
- Antibody protocols
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Accessories
10 % until end of 2023. Use discount code: Conj10
AS10 1197 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG