Goat anti-Human IgG Fc, ALP conjugated, min. cross-reactivity to bovine/mouse/rabbit serum

AS10 793 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG Fc (two heavy chains with constant domains)

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product information
Background		Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Immunogen		Purified human IgG
Host		Goat
Clonality		Polyclonal
Clone
Purity		Affinity purified goat IgG
Format		Liquid
Quantity		1 mg
Reconstitution
Storage		Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications		ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Related products		other secondary antibodies to human immunoglobulins
Additional information		ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

application information
Recommended dilution		1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Expected \| apparent MW
Confirmed reactivity		Human IgG Fc (two heavy chains with constant domains)
Predicted reactivity		Human IgG Fc (two Heavy chains with constant domains)
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		This antibody reacts with the heavy (gamma) chains on human IgG based on immunoelectrophoresis. No reactivity is observed with the light chains on human immunoglobulins, human IgG F(ab)' fragment or non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Selected references		to be added when available

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