Goat anti-Human IgG Fc, ALP conjugated, min, cross-reactivity to human IgA+IgM
AS10 800 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG Fc (two heavy chains with constant domains)
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Product Information
Immunogen
Purified human IgG
Host
Goat
Clonality
Polyclonal
Purity
Immunogen affinity purified goat IgG.
Format
Liquid
Quantity
1 mg
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Tested applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Reactivity
Confirmed reactivity
Human IgG Fc (two heavy chains with constant domains)
Predicted reactivity
Human IgG Fc (two Heavy chains with constant domains)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.
No reactivity is observed with the light chains on human immunoglobulins and to human IgA or IgM based on immunoelectrophoresis.
Background
Background
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product citations
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