Goat anti-Human IgG Fc, ALP conjugated, min, cross-reactivity to human IgA+IgM

Product no: AS10 800

AS10 800 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Human IgG Fc (two heavy chains with constant domains)

10 % until end of 2023. Use discount code: Conj10

233
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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    Purified human IgG

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Liquid
    Quantity: 1 mg
    Storage: Non-diluted antibody is stable for 4 years at 2-8C. For storage at -20C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
  • Reactivity
  • Confirmed reactivity: Human IgG Fc (two heavy chains with constant domains)
    Predicted reactivity: Human IgG Fc (two Heavy chains with constant domains)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
    Additional information (application):

    This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.

    No reactivity is observed with the light chains on human immunoglobulins and to human IgA or IgM based on immunoelectrophoresis.

  • Background
  • Background:

    Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.

  • Protocols
  • Antibody protocols
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