Goat anti-Human IgG (H&L), ALP conjugated

AS10 762 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (heavy and light chains - H&L)

Product Information

Immunogen

Purified human IgG

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Liquid

Quantity 1 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 500-1 :2000 (ELISA) , 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Human IgG (H&L)

Predicted reactivity Human IgG (H&L)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative

This antibody reacts with alpha (heavy chains) on human IgG and all light chains on human immunoglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin human serum proteins based in immunoelectrophoresis.

Background

Goat anti-human IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.

Product citations

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