Goat anti-Human IgG (H&L), ALP conjugated
AS10 762 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (heavy and light chains - H&L)
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Product Information
Immunogen
Purified human IgG
Host
Goat
Clonality
Polyclonal
Purity
Affinity purified goat IgG
Format
Liquid
Quantity
1 mg
Storage
Non-diluted antibody is stable for 4 years at 2-8°C, For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity, Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol, Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming
Tested applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 500-1 :2000 (ELISA) , 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Reactivity
Confirmed reactivity
Human IgG (H&L)
Predicted reactivity
Human IgG (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Additional information
Additional information
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
This antibody reacts with alpha (heavy chains) on human IgG and all light chains on human immunoglobulins based on immunoelectrophoresis.
No reactivity is observed to non-immunoglobulin human serum proteins based in immunoelectrophoresis.
Background
Background
Goat anti-human IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.
Product citations
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