Goat anti-Human IgG (H&L), HRP conjugated
AS10 764 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (heavy and light chains, H&L)
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Product Information
Immunogen
Purified human IgG, whole molecule
Host
Goat
Clonality
Polyclonal
Purity
Immunogen affinity purified goat IgG.
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Storage
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
The optimal working dilution should be determined by the investigator
Reactivity
Confirmed reactivity
Heavy chains on human IgG and light chanins on all human immunoglobulins
Not reactive in
Non immunoglobulin human serum proteins
Application examples
Additional information
Additional information
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free with 0,1 % (v/v) of ProClin 150 is used as preservative
Background
Background
Goat anti-human IgG (H&L) is a secondary antibody conjugated to HRP which binds to human IgG (H&L) in immunological assays.
Product citations
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