Goat anti-Human IgG (H&L), HRP conjugated

AS10 764 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (heavy and light chains, H&L)

Product Information

Immunogen

Purified human IgG, whole molecule

Host Goat

Clonality Polyclonal

Purity Affinity purified using solid phase human IgG

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.

Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution The optimal working dilution should be determined by the investigator.

Reactivity

Confirmed reactivity Heavy chains on human IgG and light chanins on all human immunoglobulins

Not reactive in Non immunoglobulin human serum proteins.

Application examples

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free with 0.1 % (v/v) of ProClin 150 is used as preservative.