Goat anti-Human IgG (H&L), HRP conjugated, min, cross-reactivity bovine/mouse/rabbit serum
AS10 772 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (heavy and light chains, H&L)
10 % until end of 2023. Use discount code: Conj10
- Product Info
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Immunogen: Purified human IgG
Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: The optimal working dilution should be determined by the investigator - Reactivity
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Confirmed reactivity: Human IgG (H&L) Predicted reactivity: Human IgG (H&L) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Additional information (application): This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunglobulins based on immunoelectrophoresis.
No reactivity is observed to non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
- Background
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Background: Goat anti-human IgG (H&L) is a secondary antibody conjugated to HRP which binds to human IgG (H&L) in immunological assays.
- Protocols
- Antibody protocols
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Accessories
AS10 1089 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (H&L)
AS10 1124 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (H&L)
AS10 1039 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG + IgA + IgM
10 % until end of 2023. Use discount code: Conj10