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Goat anti-Human IgG (H&L), HRP conjugated, min. cross-reactivity bovine/mouse/rabbit serum

AS10 772 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Human IgG (heavy and light chains, H&L)

Goat anti-Human IgG (H&L), HRP conjugated, min. cross-reactivity bovine/mouse/rabbit serum in the group Secondary Antibodies / Anti-Human / HRP (horse radish peroxidase) / IgG at Agrisera AB (Antibodies for research) (AS10 772)

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Product Information

Immunogen

Purified human IgG

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution The optimal working dilution should be determined by the investigator.

Reactivity

Confirmed reactivity Human IgG (H&L)
Predicted reactivity Human IgG (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.

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Background

Background

Goat anti-human IgG (H&L) is a secondary antibody conjugated to HRP which binds to human IgG (H&L) in immunological assays.

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Selected references

to be added when available