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Goat anti-Human IgG (H&L), ALP conjugated, min. cross-reactivity to bovine/mouse/rabbit serum

AS10 768 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Human IgG (heavy and light chains, H&L)

Goat anti-Human IgG (H&L), ALP conjugated, min. cross-reactivity to bovine/mouse/rabbit serum in the group Secondary Antibodies / Anti-Human / ALP (alkaline phosphatase) / IgG at Agrisera AB (Antibodies for research) (AS10 768)

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Product Information

Immunogen

Purified human IgG

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Liquid
Quantity 1 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Human IgG (H&L)
Predicted reactivity Human IgG (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

This antibody reacts with the heavy (gamma) chains on human IgG and all lights chains of human immunoglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin human serum proteins and serum proteins from mouse, rabbit or bovine based on immunoelectrophoresis.

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Background

Background

Goat anti-human IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.

Product citations

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