showForm Goat anti-human IgM (ĩ chain), F(ab)'2 fragment, ALP (alkaline

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Goat anti-Human IgM (ĩ chain), F(ab)'2 fragment, ALP conjugated

AS10 1237   | Clonality: Polyclonal  |  Host:Goat   |  Reactivity:Human IgM (µ chain)

Goat anti-Human IgM (ĩ chain), F(ab)'2 fragment, ALP conjugated  in the group Secondary Antibodies / Anti-Human / ALP (alkaline phosphatase) / IgM at Agrisera AB (Antibodies for research) (AS10 1237)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified IgG, F(ab)'2 fragment
Format Liquid
Quantity 0.5 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)

Reactivity

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Additional information

Additional information

This antibody reacts with:heavy (µ) chains on human IgM based on immunoelectrophoresis.

Minimum cross-reactivity is observed to: non-immunoglobulin serum proteins human IgG.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

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Background

Background Goat anti-human IgM (µ chain), ALP (alkaline phosphatase) conjugated is a F(ab)'2 fragment of a secondary antibody which binds to human IgM in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

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