showForm Goat anti-human IgM, ĩ chain,HRP conjugated

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Goat anti-Human IgM (ĩ chain), HRP conjugated

AS10 1035 | Clonality: Polyclonal  |  Host: Goat  |  Reactivity: Human IgM

Goat anti-Human IgM (ĩ chain), HRP conjugated in the group Secondary Antibodies / Anti-Human / HRP (horse radish peroxidase) / IgM at Agrisera AB (Antibodies for research) (AS10 1035)

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Product name, number (Agrisera, Sweden)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on human IgM based on immunoelectrophoresis. Minimum cross-reactivity is observed with IgG, F(ab)'2 fragment based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.

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Background

Background Goat anti-human IgM, µ chain,HRP conjugated is a secondary antibody which binds to human IgM in immunological assays. Antibodies affinity purified using solid phase human IgM.

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