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Goat anti-Human kappa chain, Biotin conjugated

AS10 1404 | Clonality: Polyclonal | Host: Goat | Reactivity: Human kappa chain
Goat anti-Human kappa chain, Biotin conjugated in the group Secondary Antibodies / Anti-Human / Biotinylated / IgA at Agrisera AB (Antibodies for research) (AS10 1404)

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Product Information

Immunogen Purified human Kappa (k) Chain BAA37169.1
Host Goat
Clonality Polyclonal
Purity Affinity purified IgG
Format Liquid
Quantity 0.5 mg
Storage Store at 2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500 - 1 : 5 000 (IHC)

Reactivity

Application examples

Additional information

Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.

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Background

Background Goat anti-human kappa (k) chain is a secondary antibody conjugated to biotin, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.

Product citations

Selected references Reinhart et al. (2014). In search of expression bottlenecks in recombinant CHO cell lines-a case study. Appl Microbiol Biotechnol. 2014 Feb 21.

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