Goat anti-Human kappa chain, HRP conjugated

Name: Goat anti-Human kappa chain, HRP conjugated
SKU: AS10 1406
Price: 253 EUR
Availability: InStock

Product no: AS10 1406

AS10 1406 | Clonality: Polyclonal; | Host: Goat | Reactivity: Human kappa chain

253 €

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DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	Purified human Kappa (k) Light Chain BAA37169.1
Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified goat IgG using solid phase Human Kappa (κ) Light Chain
Format:	Lyophilized
Quantity:	0.5 mg
Reconstitution:	Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Storage:	Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications:	ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution:	1: 200 - 1: 5000 (ELISA), 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5000 (WB)

Reactivity
Confirmed reactivity: Kappa (κ) Light Chain on all human immunoglobulins
Additional Information

Additional information:

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.

Additional information (application):

Based on IEP, no reactivity is observed to:

non-immunoglobulin human serum proteins
heavy chains on all human immunoglobulins
Lambda (λ) light chains on all human immunoglobulins

Background

Background:

Goat anti-human kappa (k) chain is a secondary antibody conjugated to HRP, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.

Protocols
Antibody protocols
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