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Goat anti-Human kappa light chain, DyLightŪ 594 conjugated

AS12 2040 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Human kappa light chain

Goat anti-Human kappa light chain, DyLightŪ 594 conjugated in the group Secondary Antibodies / Anti-Human / Fluorescent / IgM at Agrisera AB (Antibodies for research) (AS12 2040)

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Product Information

Immunogen

Purified human kappa light chain

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Additional information

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on  immunoelectrophoresis, this antibody reacts with:  kappa  light chains on human immunoglobulins.

No reactivity is observed to: non-immunoglobulin human serum proteins heavy chains on human immunoglobulins lambda  light chains on human immunoglobulins.

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Background

Background

Goat anti-human kappa light chain , DyLight®594 Conjugate is a secondary antibody conjugated to  DyLight® 594, which binds to all human kappa light chain in immunological assays. 

DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase human kappa light chain.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

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