Goat anti-Human kappa light chain, DyLight® 650 conjugated

AS12 2294 | Clonality: Polyclonal | Host: Goat | Reactivity: Human kappa light chain

Product Information

Immunogen Purified Human Kappa (?) Chain

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 0,5 mg

Reconstitution For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Not reactive in

Application examples

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: kappa (к) light chains on human immunoglobulins

No reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda (к) light chains on human immunoglobulins

Background

Goat anti-human kappa light chain - DyLight®650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to human kappa light chain in immunological assays.

DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are affinity purified using solid phase Human Kappa (к) Chain

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

Picture (footer):
not reactive in:
additional information:	Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with: kappa (к) light chains on human immunoglobulins No reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda (к) light chains on human immunoglobulins
additional information (application):
background:	Goat anti-human kappa light chain - DyLight®650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to human kappa light chain in immunological assays. DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are affinity purified using solid phase Human Kappa (к) Chain DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Goat
immunogen:	Purified Human Kappa (?) Chain
Purity:	Immunogen affinity purified goat IgG.
Quantity:	0,5 mg
recommended dilution:	1 : 20-1 : 2000 for most applications
Reconstitution:	For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

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