Goat anti-Llama IgG (H&L), HRP conjugated
AS10 1420 | Clonality: Polyclonal | Host: Goat | Reactivity: Llama IgG (H&L)
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Product Information
Immunogen
Purified llama IgG (H&L), whole molecule, AAQ19986
Host
Goat
Clonality
Polyclonal
Purity
Affinity purified using solid phase llama IgG
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
Storage
Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
The optimal working dilution should be determined by the investigator.
Reactivity
Confirmed reactivity
Heavy chains on llama IgG and light chains on all llama immunoglobulins.
Not reactive in
Non-immunoglobulin llama serum proteins.
Application examples
Additional information
Additional information
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.
The antibody is not adsorbed against other species IgGs, therefore it might react with rabbit immunoglobulins.
This antibody will react with VHH of llama IgG's.
Background
Background
Goat anti-llama IgG (H&L) HRP conjugated is a secondary antibody conjugated to HRP, which binds to llama IgG (H&L) and will recognize llama VHH domain in immunological assays. Antibody is affinity purified using solid phase llama IgG (H&L).
Product citations
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