Goat anti-Mouse IgG (H&L), HRP conjugated, min, cross-reactivity to bovine, horse, human, pig or rabbit serum

Name: Goat anti-Mouse IgG (H&L), HRP conjugated, min, cross-reactivity to bovine, horse, human, pig or rabbit serum
SKU: AS10 1427
Price: 167 EUR
Availability: InStock

AS10 1427 Clonality: Polyclonal Host: Goat Reactivity: Mouse IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Goat anti-Mouse IgG (H&L), HRP conjugated, min, cross-reactivity to bovine, horse, human, pig or rabbit serum in the group Secondary Antibodies / Anti-Mouse / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 1427)

Product Information

Immunogen Purified mouse IgG (H&L) AAA51107

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1: 2000 - 1: 5000 (ELISA, WB), 1: 500 - 1: 5000 (IHC)

Reactivity

Application examples

Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit.

Background

Background Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L).

Product citations

Selected references Barahimipour et al. (2016). Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker. Plant Mol Biol. 2016 Mar;90(4-5):403-18. doi: 10.1007/s11103-015-0425-8. Epub 2016 Jan 8.

immunogen:	Purified mouse IgG (H&L) AAA51107
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified goat IgG.
Format:	Lyophilized
Quantity:	1 mg
storage:	Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1: 2000 - 1: 5000 (ELISA, WB), 1: 500 - 1: 5000 (IHC)
additional information:	Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth. Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit.
background:	Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L).
All references:	Barahimipour et al. (2016). Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker. Plant Mol Biol. 2016 Mar;90(4-5):403-18. doi: 10.1007/s11103-015-0425-8. Epub 2016 Jan 8.

Related products: Goat anti-Mouse IgG (H&L), HRP conjugated, min, cross-reactivity to bovine, horse, human, pig or rabbit serum

Goat anti-Mouse IgG (H&L), FITC conjugated, min, cross-reactivity to human IgG/serum

AS10 962 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG (H&L)

233 €

Goat anti-Mouse IgG (H&L), TRITC conjugated, min, cross-reactivity tohuman IgG/serum

AS10 1049 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG (H&...

165 €

Goat anti-Mouse IgG Fc (heavy chain), Affinity purified, Unconjugated

AS10 988 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG Fc

165 €

Goat anti-Mouse IgG Fc, Biotin conjugated

AS10 1020 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG Fc part

246 €