Goat anti-mouse IgM (µ chain), HRP conjugated, min, cross-reactivity to human IgG/serum
AS10 969 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgM, µ chain
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Product Information
Host
Goat
Clonality
Polyclonal
Purity
Immunogen affinity purified goat IgG.
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Recommended dilution
1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
Reactivity
Application examples
Additional information
Additional information
This antibody reacts with the heavy chains on mouse IgM based on immunoelectrophoresis.
Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins or to human IgG or serum proteins based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Background
Background
Goat anti-mouse IgM, µ chain is a HRP conjugated secondary antibody which binds to mouse IgM, µ chain in immunological assays. It has been specially adsorbed against human IgG/serum. Antibodies are affinity purified using solid phase mouse IgM.
Product citations
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