Goat anti-mouse IgM (ľ chain), HRP conjugated, min, cross-reactivity to human IgG/serum

Name: Goat anti-mouse IgM (ľ chain), HRP conjugated, min, cross-reactivity to human IgG/serum
Brand: 1
SKU: AS10 969
Price: 206 EUR
Availability: InStock

Product no: AS10 969

AS10 969 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgM, µ chain

10 % until end of 2023. Use discount code: Conj10

206 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified goat IgG.
Format:	Lyophilized
Quantity:	1 mg
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage:	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Recommended dilution:	1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)

Additional Information

Additional information:

This antibody reacts with the heavy chains on mouse IgM based on immunoelectrophoresis.

Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins or to human IgG or serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

Background

Background:

Goat anti-mouse IgM, µ chain is a HRP conjugated secondary antibody which binds to mouse IgM, µ chain in immunological assays. It has been specially adsorbed against human IgG/serum. Antibodies are affinity purified using solid phase mouse IgM.

Protocols
Antibody protocols
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