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Goat anti-mouse IgM (µ chain), HRP conjugated, min. cross-reactivity to human IgG/serum

AS10 969  | Clonality: Polyclonal  |  Host: Goat |  Reactivity: Mouse IgM, µ chain
Goat anti-mouse IgM (µ chain), HRP conjugated, min. cross-reactivity to human IgG/serum  in the group Secondary Antibodies / Anti-Mouse / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 969)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on mouse IgM based on immunoelectrophoresis.

Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins or to human IgG or serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

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Background

Background Goat anti-mouse IgM, µ chain is a HRP conjugated secondary antibody which binds to mouse IgM, µ chain in immunological assays. It has been specially adsorbed against human IgG/serum. Antibodies are affinity purified using solid phase mouse IgM.

Product citations

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