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Goat anti-Rabbit IgG (H&L), DyLightŪ 488 conjugated, min, cross-reactivity to bovine, goat, human, mouse, rat IgG or serum proteins

AS10 1216 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

Goat anti-Rabbit IgG (H&L), DyLightŪ 488 conjugated, min, cross-reactivity to bovine, goat, human, mouse, rat IgG or serum proteins in the group Secondary Antibodies / Anti-Rabbit / Fluorescent / DyLightŪ / DyLightŪ 488 at Agrisera AB (Antibodies for research) (AS10 1216)



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Product Information

Immunogen

Purified Rabbit IgG, whole molecule

Host Goat
Clonality Polyclonal
Purity Immunogen affinity purified goat IgG.
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Predicted reactivity
Not reactive in

Application examples

Application examples

Additional information

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulins

No reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from bovine, goat, human, mouse or rat IgG from bovine, goat, human, mouse or rat

Related products

Background

Background

Goat anti-rabbit IgG (H&L), DyLight®488 Conjugated, min. cross-reactivity to bovine, goat, human, mouse, rat IgG or serum proteins is a secondary antibody conjugated to DyLight® 488, which binds to Rabbit IgG (H&L) in immunological assays.

DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

immunogen:

Purified Rabbit IgG, whole molecule

Host: Goat
Clonality: Polyclonal
Purity: Immunogen affinity purified goat IgG.
Format: Lyophilized
Quantity: 1 mg
Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
recommended dilution: 1 : 20-1 : 2000 for most applications
predicted reactivity:
not reactive in:
Picture (footer):
additional information:

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulins

No reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from bovine, goat, human, mouse or rat IgG from bovine, goat, human, mouse or rat

additional information (application):
background:

Goat anti-rabbit IgG (H&L), DyLight®488 Conjugated, min. cross-reactivity to bovine, goat, human, mouse, rat IgG or serum proteins is a secondary antibody conjugated to DyLight® 488, which binds to Rabbit IgG (H&L) in immunological assays.

DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

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