Goat anti-Rabbit IgG (H&L), DyLightŪ 488 conjugated, min. cross-reactivity to bovine, human, mouse IgG or serum proteins
AS09 634 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
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Purified Rabbit IgG, whole molecule
Purity Affinity purified goat IgG
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 20-1 : 2000 for most applications
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulins.
No reactivity is observed to: non-immunoglobulin rabbit serum proteins, IgG from human or mouse serum proteins from bovine, human or mouse
Goat anti-rabbit IgG (H&L), DyLight®488 Conjugated, min. cross-reactivity to bovine, human, mouse IgG or serum proteins is a secondary antibody conjugated to DyLight® 488, which binds to Rabbit IgG (H&L) in immunological assays.
DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L).
DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
To be added when available