Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, ALP conjugated

AS10 848 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)


product information
Background		Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) immunological assays.
Immunogen		Purified Rabbit IgG
Host		Goat
Clonality		Polyclonal
Clone
Purity		Affinity purified goat IgG, F(ab)'2 fragment
Format		Liquid
Quantity		0.5 mg
Reconstitution
Storage		Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications		ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Related products		other antibodies to rabbit IgG
Additional information		ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative. Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.

application information
Recommended dilution		1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Expected \| apparent MW
Confirmed reactivity		Rabbit IgG (H&L)
Predicted reactivity		Rabbit IgG (H&L)
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Selected references		to be added when available

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