Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated, min, cross-reactivity to bovine,human, mouse IgG/serum

Product no: AS10 1146

AS10 1146   | Clonality: Polyclonal  |  Host: Goat   |  Reactivity: Rabbit IgG (H&L)

246
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgG, F(ab)'2 fragment.
    Format: Lyophilized
    Quantity: 0,5 mg
    Reconstitution: For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Recommended dilution: 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
  • Additional Information
  • Additional information:

    This antibody reacts with:heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

    Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from bovine, human or mouse IgG from human or mouse.

    Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

  • Background
  • Background: Goat anti-rabbit IgG (H&L),  HRP conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to rabbit IgGs in immunological assays. Antibodies are adsorbed against bovine,human,mouse IgG/serum and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
  • Product Citations
  • Selected references: Banday and Lajon (2017). Elevated systemic glutamic acid level in the non-obese diabetic mouse is Idd linked and induces beta cell apoptosis. Immunology. 2017 Feb;150(2):162-171. doi: 10.1111/imm
  • Protocols
  • Antibody protocols
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (1)
    Viqar Banday | 2020-01-30
    0 | 0
    This antibody works well for western blotting assay. I detected multiple antigens in the blot ranging from 36kDa to 66kDa. The result looks great with this antibody (1:10,000 dilution).

Accessories

AS10 1230 | Clonality: Polyclonal  |  Host:Goat   |  Reactivity: Rabbit IgG

246 €
Add to cart
Info

AS10 1196 | Clonality: Polyclonal  |  Host: Goat   |  Reactivity: Rabbit IgG (H&L)

https://www.agrisera.com/en/artiklar/animal-cell-biology/secondary-antibodies-2/anti-Sheep-igg-secondary-antibodies-2/hrp-alkaline-phosphatase-9/index.html
162 €
Add to cart
Info

AS10 1233 | Clonality: Polyclonal  |  Host:Goat   |  Reactivity: Rabbit IgG (H&L)

175 €
Add to cart
Info

AS10 1086  | Clonality: Polyclonal  |  Host: Goat  |  Reactivity: Rabbit IgG (H&L)

260 €
Add to cart
Info
Close