Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated, min, cross-reactivity to bovine,human, mouse IgG/serum

Name: Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated, min, cross-reactivity to bovine,human, mouse IgG/serum
SKU: AS10 1146
Price: 233 EUR
Availability: InStock
Rating: 5 (1 reviews)

AS10 1146 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated, min, cross-reactivity to bovine,human, mouse IgG/serum in the group Secondary Antibodies / Anti-Rabbit / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 1146)

Product Information

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified IgG, F(ab)'2 fragment.

Format Lyophilized

Quantity 0,5 mg

Reconstitution For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Recommended dilution 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)

Reactivity

Application examples

Additional information

This antibody reacts with:heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from bovine, human or mouse IgG from human or mouse.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

Background

Background Goat anti-rabbit IgG (H&L), HRP conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to rabbit IgGs in immunological assays. Antibodies are adsorbed against bovine,human,mouse IgG/serum and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

Product citations

Selected references Banday and Lajon (2017). Elevated systemic glutamic acid level in the non-obese diabetic mouse is Idd linked and induces beta cell apoptosis. Immunology. 2017 Feb;150(2):162-171. doi: 10.1111/imm

Clonality:	Polyclonal
Format:	Lyophilized
Host:	Goat
Purity:	Immunogen affinity purified IgG, F(ab)'2 fragment.
Quantity:	0,5 mg
recommended dilution:	1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
Reconstitution:	For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
background:	Goat anti-rabbit IgG (H&L), HRP conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to rabbit IgGs in immunological assays. Antibodies are adsorbed against bovine,human,mouse IgG/serum and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
additional information:	This antibody reacts with:heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from bovine, human or mouse IgG from human or mouse. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
All references:	Banday and Lajon (2017). Elevated systemic glutamic acid level in the non-obese diabetic mouse is Idd linked and induces beta cell apoptosis. Immunology. 2017 Feb;150(2):162-171. doi: 10.1111/imm

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