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Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, FITC conjugated

AS10 1011   | Clonality: Polyclonal  |  Host: Goat  |  Reactivity: Rabbit IgG (H&L)

Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, FITC conjugated  in the group Secondary Antibodies / Anti-Rabbit / Fluorescent / FITC, TRITC at Agrisera AB (Antibodies for research) (AS10 1011)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified IgG, F(ab)'2 fragment
Format Liquid
Quantity 0.5 mg
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Recommended dilution 1 : 50-1 : 5000 (ICC) (Flow cyt), 1 : 20-1 : 2000 (IHC)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

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Background

Background Goat anti-rabbit IgG (H&L), F(ab)'2 fragment of FITC (fluorescein-5-isothiocyanate) conjugated secondary antibody which binds to rabbit IgG in immunological assays. FITC has  Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is >90% based on SDS-PAGE and is affinity purified on solid phase rabbit IgG. Antibody solution may contain small amounts of intact IgG.

Product citations

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