Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, FITC conjugated, min. cross-reactivity to bovin, human, mouse IgG/serum
AS10 1042 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

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Product Information
Host
Goat
Clonality
Polyclonal
Purity
Affinity purified IgG, F(ab)'2 fragment
Format
Liquid
Quantity
0.5 mg
Storage
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Recommended dilution
1 : 50-1 : 5000 (ICC) (Flow cyt), 1 : 20-1 : 2000 (IHC)
Reactivity
Application examples
Additional information
Additional information
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Background
Background
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment, is FITC (fluorescein-5-isothiocyanate) conjugated secondary antibody which binds to all rabbit IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed against bovine, human,mouse IgG/serum and its purity is is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product citations
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