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Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated

AS10 852 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Rabbit IgG (H&L)

Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated  in the group Human/Animal Antibodies / Secondary Antibodies/Blocking / Anti-rabbit  / HRP (horseradish peroxidase) at Agrisera AB (Antibodies for research) (AS10 852)

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product information
Background

Goat anti-rabbit IgG (H&L), F(ab)'2 fragment  is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.

Immunogen

Purified Rabbit IgG (H&L)

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG, F(ab)'2 fragment
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Related products

other antibodies to rabbit IgG HRP conjugated

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.

application information
Recommended dilution The optimal working dilution should be determined by the investigator.
Expected | apparent MW
Confirmed reactivity Rabbit IgG (H&L)
Predicted reactivity Rabbit IgG (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

Selected references Linster et al. (2015). Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun. 2015 Jul 17;6:7640. doi: 10.1038/ncomms8640.


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