Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, TRITC conjugated

AS10 1018 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

Product Information

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified IgG, F(ab)'2 fragment.

Format Liquid

Quantity 0,5 mg

Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.

Recommended dilution The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

Background

Background Goat anti-rabbit IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated F(ab)'2 fragment is a secondary antibody which binds to all rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG and is affinity purified on solid phase rabbit IgG.

Product citations

Selected references Feng et al. (2020). Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy. BMC Cancer. 2020 Jul 16;20(1):665.doi: 10.1186/s12885-020-07133-4.

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