Goat anti-Rat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG)
AS09 618 | Clonality: Polyclonal | Host: Goat | Reactivity: Rat IgG (H&L)
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Product Information
Immunogen
Purified Rat IgG, whole molecule
Host
Goat
Clonality
Polyclonal
Purity
Purified goat IgG
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
Storage
Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
Reactivity
Confirmed reactivity
Rat IgG heavy and light chains (H&L) of all Rat immunoglobulins
Predicted reactivity
Rat IgG Heavy and Light chains (H&L) of all Rat immunoglobulins
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative.
No reactivity is observed to non-immunoglobulin rat serum. Antibody has been absorbed against mouse IgG and no reactivity is observed to human or mouse IgG based on immunoelectrophoresis.
Background
Background
Goat anti-rat IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Product citations
Selected references
to be added when available
Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | Fax: +46(0)935 33044 | 
