Goat anti-Rat IgG (H&L), HRP conjugated, min, cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG)

AS09 618 | Clonality: Polyclonal | Host: Goat | Reactivity: Rat IgG (H&L)

Product Information

Immunogen

Purified Rat IgG, whole molecule

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily

Storage Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)

Reactivity

Confirmed reactivity Rat IgG heavy and light chains (H&L) of all Rat immunoglobulins

Predicted reactivity Rat IgG Heavy and Light chains (H&L) of all Rat immunoglobulins

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

No reactivity is observed to non-immunoglobulin rat serum, Antibody has been absorbed against mouse IgG and no reactivity is observed to human or mouse IgG based on immunoelectrophoresis

Background

Goat anti-rat IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.

Product citations

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