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Epitope tag Antibodies



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Goat anti-Rat IgG (H&L), TRITC conjugated

AS10 1266 | Clonality: Polyclonal | Host:Goat | Reactivity:Rat IgG (H&L)

Goat anti-Rat IgG (H&L), TRITC conjugated in the group Human/Animal Antibodies / Secondary Antibodies/Blocking / Anti-rat / Fluorescent / FITC, TRITC at Agrisera AB (Antibodies for research) (AS10 1266)

PRODUCT INFORMATION IN PDF

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product information
Background Goat anti-rat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to rat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase rat IgG.
Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products other TRITC conjugated secondary antibodies to rat IgG
Additional information

This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis. Please include 5 % goat serum in secondary antibody diluent buffer. 

Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
application information
Recommended dilution The optimal working dilution should be determined by the investigator. Usually 1 : 100-1 : 500.
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references

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