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Goat anti-Rat IgG (H&L), HRP conjugated

AS10 1187   | Clonality: Polyclonal  |  Host: Goat   |  Reactivity: Rat IgG (H&L)


Goat anti-Rat IgG (H&L), HRP conjugated in the group Secondary Antibodies / Anti-Rat / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 1187)

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Product name, number (Agrisera, Sweden)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified using solid phase rat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.!!AIR8!!Store liquid material at 2-8°C upto 6 month.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution The optimal working dilution should be determined by the investigator.

Reactivity

Confirmed reactivity Heavy chains on rat IgG and with the light chains on all rat immunoglobulins.
Not reactive in Non-immunoglobulin rat serum proteins.

Application examples

Additional information

Additional information

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG as preservative.

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Background

Background Goat anti-rat IgG (H&L), HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.

Product citations

Selected references Saintenac et al. (2021) A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch. Nat Commun. 2021 Jan 19;12(1):433. doi: 10.1038/s41467-020-20685-0. PMID: 33469010; PMCID: PMC7815785.

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