Goat anti-Rat IgG (H&L), HRP conjugated

Product no: AS10 1187

AS10 1187 | Clonality: Polyclonal  |  Host: Goat   |  Reactivity: Rat IgG (H&L)


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  • Product Info
  • Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified rat IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.!!AIR8!!Store liquid material at 2-8C upto 6 months.
    Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: The optimal working dilution should be determined by the investigator
  • Reactivity
  • Confirmed reactivity: Heavy chains on rat IgG and with the light chains on all rat immunoglobulins
    Not reactive in: Non-immunoglobulin rat serum proteins
  • Additional Information
  • Additional information:

    Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

    Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG as preservative.

  • Background
  • Background: Goat anti-rat IgG (H&L), HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
  • Product Citations
  • Selected references: Saintenac et al. (2021) A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch. Nat Commun. 2021 Jan 19;12(1):433. doi: 10.1038/s41467-020-20685-0. PMID: 33469010; PMCID: PMC7815785.
  • Protocols
  • Antibody protocols
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
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    Number of reviews: (1)
    Szymon Kubala | 2017-04-25
    0 | 0
    this antibody was used against primary RAT anti HA antibody. The 1:5000 dilution was performed. WB analysis was performed on the plant extract carrying the HA tagged protein

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