Goat anti-Rat IgG (H&L), HRP conjugated

Name: Goat anti-Rat IgG (H&L), HRP conjugated
SKU: AS10 1187
Price: 153 EUR
Availability: InStock
Rating: 5 (1 reviews)

AS10 1187 | Clonality: Polyclonal | Host: Goat | Reactivity: Rat IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Goat anti-Rat IgG (H&L), HRP conjugated in the group Secondary Antibodies / Anti-Rat / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 1187)

Product Information

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified rat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.!!AIR8!!Store liquid material at 2-8°C upto 6 months.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution The optimal working dilution should be determined by the investigator

Reactivity

Confirmed reactivity Heavy chains on rat IgG and with the light chains on all rat immunoglobulins

Not reactive in Non-immunoglobulin rat serum proteins

Application examples

Additional information

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG as preservative.

Background

Background Goat anti-rat IgG (H&L), HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.

Product citations

Selected references Saintenac et al. (2021) A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch. Nat Commun. 2021 Jan 19;12(1):433. doi: 10.1038/s41467-020-20685-0. PMID: 33469010; PMCID: PMC7815785.

Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified rat IgG.
Format:	Lyophilized
Quantity:	1 mg
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.!!AIR8!!Store liquid material at 2-8°C upto 6 months.
Tested applications:	ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	The optimal working dilution should be determined by the investigator
Confirmed reactivity:	Heavy chains on rat IgG and with the light chains on all rat immunoglobulins
not reactive in:	Non-immunoglobulin rat serum proteins
additional information:	Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0) Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG as preservative.
background:	Goat anti-rat IgG (H&L), HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
All references:	Saintenac et al. (2021) A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch. Nat Commun. 2021 Jan 19;12(1):433. doi: 10.1038/s41467-020-20685-0. PMID: 33469010; PMCID: PMC7815785.

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