Goat anti-Rat IgG (H&L), ALP conjugated, min. reactivity to Human and mouse IgG, highly adsorbed against mouse IgG

AS10 663 | Clonality: Polyclonal | Host: Goat | Reactivity: Rat IgG (H&L)

Product Information

Immunogen

Purified Rat IgG, whole molecule

Host Goat

Clonality Polyclonal

Purity Purified goat IgG

Format Liquid

Quantity 1 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Heavy chains on Rat IgG and with the light chains on all Rat immunoglobulins based on IEP.

Predicted reactivity Heavy chains on Rat IgG and with the Light chains on all Rat immunoglobulins based on IEP.

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

APL conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

This antibody is highly cross absorbed against mouse IgG. No reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis. No reactivity is observed to human or mouse IgG based on immunoelecrophoresis.

Background

Goat anti-rat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.

Product citations

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