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Goat anti-Rat IgG (H&L), ALP conjugated

AS09 620 | Clonality: Polyclonal | Host: Goat | Reactivity: Rat IgG (H&L)

Goat anti-Rat IgG (H&L), ALP conjugated  in the group Secondary Antibodies / Anti-Rat / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS09 620)

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Product Information

Immunogen

Purified Rat IgG, whole molecule

Host Goat
Clonality Polyclonal
Purity Purified goat IgG
Format Liquid
Quantity 1 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Rat IgG heavy and light chains (H&L)
Predicted reactivity Rat IgG Heavy and Light chains (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

No reactivity is observed to non-immunoglobulin rat serum proteins based in immunoelectrophoresis. No reacticity to mouse and human serum proteins based on immunoelectrophoresis.

Related products

Related products

AS09 618 | Goat anti-rat IgG (H&L) HRP conjugated

AS09 619 | Goat anti-rat IgG (H&L) biotin conjugated

Background

Background

Goat anti-rat IgG is a secondary antibody conjugated to ALP (alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.

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