Goat anti-Rat IgG (H&L), ALP conjugated
AS09 620 | Clonality: Polyclonal | Host: Goat | Reactivity: Rat IgG (H&L)
10 % until end of 2023. Use discount code: Conj10
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Purified Rat IgG, whole molecule
Purity Immunogen affinity purified goat IgG.
Quantity 1 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Confirmed reactivity Rat IgG heavy and light chains (H&L)
Predicted reactivity Rat IgG Heavy and Light chains (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known
Additional information ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
No reactivity is observed to non-immunoglobulin rat serum proteins based in immunoelectrophoresis, No reacticity to mouse and human serum proteins based on immunoelectrophoresis
Goat anti-rat IgG is a secondary antibody conjugated to ALP (alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.
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