Goat anti-Rat IgG (H&L), F(ab)'2 fragment, DyLight® 488 conjugated, min, cross-reactivity to human, mouse lgG

Product no: AS10 1217

AS10 1217 | Clonality: Polyclonal | Host: Goat | Reactivity: Rat IgG (H&L) - F(ab)'2 fragment

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  • Product Info
  • Immunogen:

    Purified Rat IgG, whole molecule

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Lyophilized
    Quantity: 0,5 mg
    Reconstitution: For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Recommended dilution: 1 : 20-1 : 2000 for most applications
  • Reactivity
  • Predicted reactivity: ,
    Not reactive in:
  • Application Examples
  • Additional Information
  • Additional information:

    Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

    Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulins

    No reactivity is observed to: non-immunoglobulin mouse serum proteins, IgG from human or mouse

    Additional information (application):
  • Background
  • Background:

    Goat anti-Rat IgG (H&L) - F(ab)'2 fragment, DyLight488 Conjugated, min. cross-reactivity to human, mouse lgG is a secondary antibody conjugated to DyLight® 488, which binds to Rat IgG (H&L) - F(ab)'2 fragment in immunological assays.

    DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rat IgG (H&L)

    DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Antibody protocols
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