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Goat anti-Rat IgG (H&L), F(ab)'2 fragment, HRP conjugated, min.reactivity to human, mouse IgG

AS10 1145   | Clonality: Polyclonal  |  Host:Goat   |  Reactivity:Rat IgG (H&L)

Goat anti-Rat IgG (H&L), F(ab)'2 fragment, HRP conjugated, min.reactivity to human, mouse IgG  in the group Secondary Antibodies / Anti-Rat / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 1145)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified IgG, F(ab)'2 fragment
Format Liquid
Quantity 0.5 mg
Storage Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution The optimal working dilution should be determined by the investigator.

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from human or mouse.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

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Background

Background Goat anti-rat IgG (H&L),  HRP conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to rat IgGs in immunological assasys. Antibodies are adsorbed against human,mouse IgG and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

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