Goat anti-Rat IgG (H&L), TRITC conjugated, min, cross-reactivity to human, mouse IgG

AS10 1152 | Clonality: Polyclonal | Host: Goat | Reactivity: Rat IgG (H&L)

Product Information

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Recommended dilution 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or to human or mouse IgG based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

Background

Background Goat anti-rat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human,mouse IgG and affinity purified using solid phase rat IgG.

Product citations

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background:	Goat anti-rat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human,mouse IgG and affinity purified using solid phase rat IgG.
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Goat
Purity:	Immunogen affinity purified IgG.
Quantity:	1 mg
recommended dilution:	1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
additional information:	This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or to human or mouse IgG based on immunoelectrophoresis. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.