Anti-GST tag (mouse monoclonal) (clone S-tag-05)

Name: Anti-GST tag (mouse monoclonal) (clone S-tag-05)
SKU: AS21 4621
Price: 282 EUR
Availability: InStock

Product no: AS21 4621

AS21 4621 | Clonality: Monoclonal | Host: Mouse | Reactivity: GST-tagged proteins

282 €

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DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	GST protein
Sub class:	IgG2b
Host:	Mouse
Clonality:	Monoclonal
Purity:	Immunoglobulin purified on Protein A.
Format:	Liquid at 1 mg/ml.
Quantity:	0.1 mg
Storage:	Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunoprecipitation (IP), Western blot (WB)
Recommended dilution:	1 µg/ml (WB)

Reactivity
Confirmed reactivity: GST fusion proteins
Application Examples

Western blot using anti-GST tag monoclonal antibodies

Samples:
Lane 1: affinity purification of Glutathione-S-Transferase (GST) overexpressed in E. coli, using commercial GST sorbent
Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared with anti-GST monoclonal antibodies

Additional Information

Additional information:

This antibody is recognizing native and denatured fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems. It can be also used for immuno purification of GST-tagged proteins.

Background

Background:

GST-tag (glutathione S-transferase) from a parasite Schistosoma japonicum is a tag added to a protein of interest as a fusion protein for protein purification and detection. It allows purification by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.

Protocols
Agrisera Western Blot protocol and video tutorials

Antibody Protocols
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