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H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade)

AS16 3196 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, Human, Mouse, O. sativa
H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade) in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3196)
H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade)



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Product Information

Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum.
Format Liquid
Quantity 50 ĩg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Chromatin immunoprecipitation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)
Recommended dilution 0,5-5 ĩg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 4000 (ELISA), 1 : 500 (IF), 1 : 10 000 (PA), 1 : 1000 (WB)
Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Arabidopsis thaliana, Mouse, Oryza sativa
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

western blot using anti-H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade) polyclonal antibodies
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). Anti-H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade) antibodies were diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.

Additional information

Additional information Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300
Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300

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Background

Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K36 is associated with active genes.

Product citations

Picture (footer): application example

western blot using anti-H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade) polyclonal antibodies
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). Anti-H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade) antibodies were diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.
additional information: Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300
additional information (application): Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300
Confirmed reactivity: Human
predicted reactivity: Arabidopsis thaliana, Mouse, Oryza sativa
not reactive in: No confirmed exceptions from predicted reactivity are currently known
calculated | apparent molecular mass [kDa]:

15 kDa

Clonality: Polyclonal
Format: Liquid
Host: Rabbit
immunogen: KLH-conjugated synthetic peptide
Purity: Immunogen affinity purified serum.
Quantity: 50 ĩg
recommended dilution: 0,5-5 ĩg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 4000 (ELISA), 1 : 500 (IF), 1 : 10 000 (PA), 1 : 1000 (WB)
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Chromatin immunoprecipitation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)
background: Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K36 is associated with active genes.

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