H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade)
AS16 3196 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, Human, Mouse, O. sativa

Data sheet | Product citations | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum.
Format
Liquid
Quantity
50 ĩg
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Chromatin immunoprecipitation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)
Recommended dilution
0,5-5 ĩg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 4000 (ELISA), 1 : 500 (IF), 1 : 10 000 (PA), 1 : 1000 (WB)
Expected | apparent MW
15 kDa
Reactivity
Confirmed reactivity
Human
Predicted reactivity
Arabidopsis thaliana, Mouse, Oryza sativa
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example

Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). Anti-H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade) antibodies were diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.

Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). Anti-H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade) antibodies were diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.
Additional information
Additional information
Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300
Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300
Background
Background
Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K36 is associated with active genes.
Product citations
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