H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade)

AS16 3196 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, Human, Mouse, O. sativa

H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade)

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum.

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Chromatin immunoprecipitation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)

Recommended dilution 0,5-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 4000 (ELISA), 1 : 500 (IF), 1 : 10 000 (PA), 1 : 1000 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Arabidopsis thaliana, Mouse, Oryza sativa

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). Anti-H3K36me3 | Histone H3 trimethylated lysine 36 (ChIP grade) antibodies were diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.

Additional information

Additional information Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300

Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300

Background

Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K36 is associated with active genes.

Product citations

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Picture (footer):	application example Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). Anti-H3K36me3 \| Histone H3 trimethylated lysine 36 (ChIP grade) antibodies were diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.
additional information:	Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300
additional information (application):	Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300
Confirmed reactivity:	Human
predicted reactivity:	Arabidopsis thaliana, Mouse, Oryza sativa
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
calculated \| apparent molecular mass [kDa]:	15 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide
Purity:	Immunogen affinity purified serum.
Quantity:	50 µg
recommended dilution:	0,5-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 4000 (ELISA), 1 : 500 (IF), 1 : 10 000 (PA), 1 : 1000 (WB)
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Chromatin immunoprecipitation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)
background:	Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K36 is associated with active genes.