H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade)

AS16 3195 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, Human, Mouse, Rat

H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade)

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum.

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Chromatin immunoprecipitation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)

Recommended dilution 0,5-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 500 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Arabidopsis thaliana, Mouse, Rat

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

Dot blot using anti-H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade) polyclonal antibodies

Dot blot was used to to test the cross reactivity of anti-H3K27ac antibodies. Peptides containing other histone modifications and the unmodified H3K27 were used. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20 000. Results show a high specificity of the antibody for the modification of interest.

Additional information

Additional information Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300

Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300

Background

Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.

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background:	Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.
additional information:	Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300
additional information (application):	Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300
Confirmed reactivity:	Human
predicted reactivity:	Arabidopsis thaliana, Mouse, Rat
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example Dot blot was used to to test the cross reactivity of anti-H3K27ac antibodies. Peptides containing other histone modifications and the unmodified H3K27 were used. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20 000. Results show a high specificity of the antibody for the modification of interest.
calculated \| apparent molecular mass [kDa]:	15 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide
Purity:	Immunogen affinity purified serum.
Quantity:	50 µg
recommended dilution:	0,5-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 500 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Chromatin immunoprecipitation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)