H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade)
AS16 3195 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, Human, Mouse, Rat
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum in PBS with 0.05 % sodium azide
Format
Liquid
Quantity
50 µg
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Chromatin immunoprecipitation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)
Recommended dilution
0.5-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 500 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)
Expected | apparent MW
15 kDa
Reactivity
Confirmed reactivity
Human
Predicted reactivity
Arabidopsis thaliana, Mouse, Rat
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
application example
Dot blot was used to to test the cross reactivity of anti-H3K27ac antibodies. Peptides containing other histone modifications and the unmodified H3K27 were used. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20 000. Results show a high specificity of the antibody for the modification of interest.
Dot blot was used to to test the cross reactivity of anti-H3K27ac antibodies. Peptides containing other histone modifications and the unmodified H3K27 were used. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20 000. Results show a high specificity of the antibody for the modification of interest.
Additional information
Additional information
Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
Antibody solution contains PBS with 0.05% sodium azide and 0.05% ProClin 300.
Background
Background
Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.
Product citations
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