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H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade)
Product no: AS16 3195
AS16 3195 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, Human, Mouse, Rat
495
€
Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated synthetic peptide Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum. Format: Liquid Quantity: 50 µg Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Chromatin Immunoprecipitation Sequencing (ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB) Recommended dilution: 0,5-5 µg/IP (ChIP-seq), 1 : 20 000 (Dot), 1 : 500 (ELISA), 1 : 500 (IF), 1 : 1000 (WB) Expected | apparent MW: 15 kDa
- Reactivity
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Confirmed reactivity: Human Predicted reactivity: Arabidopsis thaliana, Mouse, Rat Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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application example 
Dot blot was used to to test the cross reactivity of anti-H3K27ac antibodies. Peptides containing other histone modifications and the unmodified H3K27 were used. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20 000. Results show a high specificity of the antibody for the modification of interest. - Additional Information
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Additional information: Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300 Additional information (application): Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300 - Background
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Background: Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers. - Reviews:
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