H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade)

AS16 3195 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, Human, Mouse, Rat

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS with 0.05 % sodium azide

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Chromatin immunoprecipitation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)

Recommended dilution 0.5-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 500 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Arabidopsis thaliana, Mouse, Rat

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

Dot blot using anti-H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade) polyclonal antibodies

Dot blot was used to to test the cross reactivity of anti-H3K27ac antibodies. Peptides containing other histone modifications and the unmodified H3K27 were used. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20 000. Results show a high specificity of the antibody for the modification of interest.

Additional information

Additional information Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.

Antibody solution contains PBS with 0.05% sodium azide and 0.05% ProClin 300.

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Background

Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.