IKP

H3K4me1T6p | Histone H3 (monomethylated Lys4, p Thr6)

Product no: AS16 3171
AS16 3171 Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
549
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum.
    Format: Liquid
    Quantity: 50 g
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 50 (IF), 1 : 50 (IHC), 1 : 500 (WB)
    Expected | apparent MW:

    15 kDa

  • Reactivity
  • Confirmed reactivity: Human
    Predicted reactivity: Caenorhabditis elegans, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    dot blot using anti-Histone H3 (monomethyl Lys4, p Thr6) antibodies
    Dot blot using anti-H3K4me1T6p antibodies. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

    immunofluorescence using anti- Histone H3 (monomethyl Lys4, p Thr6) antibodies
    Immunofluorescence using anti- H3K4me1T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K4me1T6p is nuclear and chromosomal. Staining: H3K4me1T6p is expressed in green and the nuclei are counterstained with DAPI (blue).

    western blot using anti-Histone H3 (monomethyl Lys4, p Thr6) antibodies

    Western Blot using anti-Rabbit H3K4me1T6p antibodies. 30 μg of C. elegans embryonic lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

    western blot using anti-Histone H3 (monomethyl Lys4, p Thr6) antibodies
    Western Blot using anti-H3K4me1T6p antibodies. 30 μg of HeLa histone extracts per lane. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
  • Additional Information
  • Additional information: This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol
  • Background
  • Background: Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).
  • Product Citations
  • Selected references: 38221900
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info
AS16 3170 Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
549 €
Add to cart
Info
AS16 3168 Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
549 €
Add to cart
Info
Close