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H3K4me1T6p | Histone H3 (monomethylated Lys4, p Thr6)

AS16 3171 Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
H3K4me1T6p | Histone H3 (monomethylated Lys4, p Thr6) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3171)

DATA SHEET IN PDF

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492
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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Liquid
Quantity 50 µg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 50 (IF), 1 : 50 (IHC), 1 : 500 (WB)
Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Caenorhabditis elegans, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

dot blot using anti-Histone H3 (monomethyl Lys4, p Thr6)  antibodies
Dot blot using anti-H3K4me1T6p antibodies. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

immunofluorescence using anti- Histone H3 (monomethyl Lys4, p Thr6) antibodies
Immunofluorescence using anti- H3K4me1T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K4me1T6p is nuclear and chromosomal. Staining: H3K4me1T6p is expressed in green and the nuclei are counterstained with DAPI (blue).

western blot using anti-Histone H3 (monomethyl Lys4, p Thr6)  antibodies

Western Blot using anti-Rabbit H3K4me1T6p antibodies. 30 μg of C. elegans embryonic lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

western blot using anti-Histone H3 (monomethyl Lys4, p Thr6) antibodies
Western Blot using anti-H3K4me1T6p antibodies. 30 μg of HeLa histone extracts per lane. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Additional information

Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.

Related products

Related products AS16 3172 | Anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodies
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AS16 3197 | Anti-anti-H3core, monoclonal antibodies
AS15 2855 | Anti-H3 | histone H3, chicken polyclonal antibodies
AS10 710 | Anti-H3 | histone H3, rabbit polyclonal antibodies
AS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710

collection of antibodies to epigenetics

Background

Background Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).

Product citations

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