H3K4me1T6p | Histone H3 (monomethylated Lys4, p Thr6)

Name: H3K4me1T6p | Histone H3 (monomethylated Lys4, p Thr6)
Brand: IKP
SKU: AS16 3171
Price: 520 EUR
Availability: InStock

AS16 3171 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.

H3K4me1T6p | Histone H3 (monomethylated Lys4, p Thr6) in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Plant Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3171)

H3K4me1T6p | Histone H3 (monomethylated Lys4, p Thr6)

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum.

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 50 (IF), 1 : 50 (IHC), 1 : 500 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Caenorhabditis elegans, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

dot blot using anti-Histone H3 (monomethyl Lys4, p Thr6) antibodies

Dot blot using anti-H3K4me1T6p antibodies. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

immunofluorescence using anti- Histone H3 (monomethyl Lys4, p Thr6) antibodies

Immunofluorescence using anti- H3K4me1T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K4me1T6p is nuclear and chromosomal. Staining: H3K4me1T6p is expressed in green and the nuclei are counterstained with DAPI (blue).

western blot using anti-Histone H3 (monomethyl Lys4, p Thr6) antibodies

western blot using anti-Histone H3 (monomethyl Lys4, p Thr6) antibodies

Western Blot using anti-Rabbit H3K4me1T6p antibodies. 30 μg of C. elegans embryonic lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

western blot using anti-Histone H3 (monomethyl Lys4, p Thr6) antibodies

Western Blot using anti-H3K4me1T6p antibodies. 30 μg of HeLa histone extracts per lane. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Additional information

Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol

Background

Background Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).

Product citations

immunogen:	KLH-conjugated synthetic peptide
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum.
Format:	Liquid
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 50 (IF), 1 : 50 (IHC), 1 : 500 (WB)
calculated \| apparent molecular mass [kDa]:	15 kDa
Confirmed reactivity:	Human
predicted reactivity:	Caenorhabditis elegans, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example Dot blot using anti-H3K4me1T6p antibodies. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT. Immunofluorescence using anti- H3K4me1T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K4me1T6p is nuclear and chromosomal. Staining: H3K4me1T6p is expressed in green and the nuclei are counterstained with DAPI (blue). Western Blot using anti-Rabbit H3K4me1T6p antibodies. 30 μg of C. elegans embryonic lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT. Western Blot using anti-H3K4me1T6p antibodies. 30 μg of HeLa histone extracts per lane. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
additional information:	This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol
background:	Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).