H3T3pK4me1 | Histone H3 (p Thr3, monomethylated Lys4)

AS16 3168 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.

H3T3pK4me1 | Histone H3 (p Thr3, monomethylated Lys4)

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum.

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000 (Dot), 1 : 100 (IF), 1 : 100 (IHC), 1 : 1000 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Caenorhabditis elegans, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

Dot blot using anti-H3T3pK4me1 polyclonal antibodies

Dot Blot using H3T3pK4me1 antibodies. Load: 1, 10, and 100 picomoles of the different peptides. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40 000 for 45 min at RT.

Immunofluorescence using H3T3pK4me1 polyclonal antibodies

Immunofluorescence using H3T3pK4me1 polyclonal antibodies

Immunofluorescence using anti-H3T3pK4me1 antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T3pK4me1 is nuclear and chromosomal. Staining: Histone H3T3pK4me1 is expressed in green while the nuclei and aplpha- tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).

Western blot using anti-H3T3pK4me1 antibodies

Western blot using anti-H3T3pK4me1 antibodies

Western Blot using anit-H3T3pK4me1 antibodies. Lane 1: HeLa histone extracts. Lane 2. NIH 3T3 histone extracts Lane 3. C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at a 1:1 000 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Additional information

Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol

Background

Background Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).

Product citations

calculated \| apparent molecular mass [kDa]:	15 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide
Purity:	Immunogen affinity purified serum.
Quantity:	50 µg
recommended dilution:	1 : 1000 (Dot), 1 : 100 (IF), 1 : 100 (IHC), 1 : 1000 (WB)
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Picture (footer):	application example Dot Blot using H3T3pK4me1 antibodies. Load: 1, 10, and 100 picomoles of the different peptides. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40 000 for 45 min at RT. Immunofluorescence using anti-H3T3pK4me1 antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T3pK4me1 is nuclear and chromosomal. Staining: Histone H3T3pK4me1 is expressed in green while the nuclei and aplpha- tubulin were coexpressed with DAPI (blue) and Dylight 550 (red). Western Blot using anit-H3T3pK4me1 antibodies. Lane 1: HeLa histone extracts. Lane 2. NIH 3T3 histone extracts Lane 3. C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at a 1:1 000 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
Confirmed reactivity:	Human
predicted reactivity:	Caenorhabditis elegans, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).
additional information:	This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol

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