H3T3pK4me1 | Histone H3 (p Thr3, monomethylated Lys4)
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Dot Blot using H3T3pK4me1 antibodies. Load: 1, 10, and 100 picomoles of the different peptides. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40 000 for 45 min at RT.
Immunofluorescence using anti-H3T3pK4me1 antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T3pK4me1 is nuclear and chromosomal. Staining: Histone H3T3pK4me1 is expressed in green while the nuclei and aplpha- tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).
Western Blot using anit-H3T3pK4me1 antibodies. Lane 1: HeLa histone extracts. Lane 2. NIH 3T3 histone extracts Lane 3. C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at a 1:1 000 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
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